Prediction of functional regions of the maize streak virus replication-associated proteins by protein-protein interaction analysis
Identifieur interne : 003B34 ( Main/Exploration ); précédent : 003B33; suivant : 003B35Prediction of functional regions of the maize streak virus replication-associated proteins by protein-protein interaction analysis
Auteurs : Gábor V. Horváth [Hongrie] ; Aladár Pettk Szandtner [Hongrie] ; Krisztina Nikovics [Hongrie] ; Metin Bilgin [Hongrie] ; Margaret Boulton [Royaume-Uni] ; Jeffrey W. Davies [Royaume-Uni] ; Crisanto Gutiérrez [Espagne] ; Dénes Dudits [Hongrie]Source :
- Plant Molecular Biology [ 0167-4412 ] ; 1998-11-01.
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Abstract
Abstract: The replication of the geminiviruses depends on the viral encoded early (complementary-sense) proteins and on host genome encoded factors. Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. These results provide new insights into the role of different regions of the MSV proteins in relation to transcriptional activation and regulation of viral DNA replication.
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DOI: 10.1023/A:1006076316887
Affiliations:
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Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. 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Horváth</name></noRegion><name sortKey="Bilgin, Metin" sort="Bilgin, Metin" uniqKey="Bilgin M" first="Metin" last="Bilgin">Metin Bilgin</name><name sortKey="Dudits, Denes" sort="Dudits, Denes" uniqKey="Dudits D" first="Dénes" last="Dudits">Dénes Dudits</name><name sortKey="Nikovics, Krisztina" sort="Nikovics, Krisztina" uniqKey="Nikovics K" first="Krisztina" last="Nikovics">Krisztina Nikovics</name><name sortKey="Pettk Szandtner, Aladar" sort="Pettk Szandtner, Aladar" uniqKey="Pettk Szandtner A" first="Aladár" last="Pettk Szandtner">Aladár Pettk Szandtner</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Boulton, Margaret" sort="Boulton, Margaret" uniqKey="Boulton M" first="Margaret" last="Boulton">Margaret Boulton</name></noRegion><name sortKey="Davies, Jeffrey W" sort="Davies, Jeffrey W" uniqKey="Davies J" first="Jeffrey W." last="Davies">Jeffrey W. Davies</name></country><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Gutierrez, Crisanto" sort="Gutierrez, Crisanto" uniqKey="Gutierrez C" first="Crisanto" last="Gutiérrez">Crisanto Gutiérrez</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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